MTech Molecular Biology And Genetic Engineering Techniques syllabus for 1 Sem 2020 scheme 20BBC14

Module-1 DNA REPLICATION 0 hours

DNA REPLICATION:

Comparative account on initiation, elongation and termination in prokaryotes and eukaryotes DNA Repair: Mismatch correction, Mechanisms in thymine-dimer repair: Photo reactivation, Nucleotide excision repair, SOS repair DNA Recombination: Homologous and nonhomologous recombination; Holliday Model; Site specific recombination: General mechanism, Examples: SSR in Bacteria bacteriophage, FLP/FRT and Cre/Lox recombination. Transcription: Prokaryotic & Eukaryotic Mechanisms; Significance of Promoters, Enhancers, Silencers, Transcription factors, Activators and repressors; Post transcriptional modifications; Transcription inhibitors

Module-2 GENETIC CODE AND ITS PROPERTIES 0 hours

GENETIC CODE AND ITS PROPERTIES:

Wobble hypothesis. Translation: Role of Ribosomes &tRNA; Mechanism of translation: Activation of amino acids, initiation complex formation, elongation of polypeptide, termination and release of polypeptide; Post-translational modifications; Transport of proteins and molecular chaperones. Transcriptional regulation in Prokaryotes: General mechanism of positive and negative control; Operon concept: lac, trp, and gal operons; Transcriptional control in Eukaryotes: Chromatin remodeling: Acetylation and deacetylation of histone proteins; Regulatory proteins: DNA binding transactivators, co-activators; Homeotic gene and their role in gene regulation.

A d v e r t i s e m e n t
Module-3 VECTORS 0 hours

VECTORS:

Plasmids, Phage Vectors, Phagemids, Cosmids, YACs and BACs; Cloning & Expression vectors. Enzymes in genetic engineering: Restriction Enzymes, DNA ligase, Klenow enzyme, T4 DNA polymerase, Polynucleotide kinase, Alkaline phosphatase. Methods in construction of recombinant vectors: Linkers, Adaptors, Homopolymeric tailing. Techniques in Genetic Engineering: Construction of libraries: Genomic and cDNA libraries. Hybridization techniques: Northern and Southern hybridizations. Polymerase Chain Reaction: General mechanism and applications; Variants of PCR; In vitro mutagenesis.

Module-4 GENE TRANSFER TECHNIQUES INTO PLANTS 0 hours

GENE TRANSFER TECHNIQUES INTO PLANTS:

Microprojectile bombardment; Agrobacterium transformation, Ti plasmid: structure and functions, Ti plasmid based vectors, mechanism of TDNA transfer; Chloroplast transformation; Transgenic science in plant improvement: resistance to biotic and abiotic stresses, bio pharming – plant s as bioreactors.

Module-5 Introduction of DNA into mammalian cells 0 hours

Introduction of DNA into mammalian cells; Animal vectors and Transfection techniques; Transgenic science for improvement of animals and livestock, animal as bioreactors for recombinant proteins. Gene transfer techniques into microbial cells: transformation, electroporation, lipofection, calcium phosphate mediated; Genetic manipulation of microbes for the production of insulin, growth hormones

 

Course outcomes:

After studying this course, students will be able to:

  • Demonstrate strong basics in principles of Molecular biology and Genetic engineering
  • Foundation to tackle live problems in various spheres of Genetic engineering

 

Question paper pattern:

The SEE question paper will be set for 100 marks and the marks scored will be proportionately reduced to 60.

  • The question paper will have ten full questions carrying equal marks.
  • Each full question is for 20 marks.
  • There will be two full questions (with a maximum of four sub questions) from each module.
  • Each full question will have sub question covering all the topics under a module.
  • The students will have to answer five full questions, selecting one full question from each module.

 

Textbook/ Textbooks

1 Molecular Biology of the Cell, 4th edition Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and PeterWalter Garland Science 2002

2 Molecular Cell Biology, 4th edition Harvey Lodish, Arnold Berk, S Lawrence Zipursky, Paul Matsudaira, David Baltimore, andJames Darnell W. H. Freeman 2000

 

Reference Books

1 Genomes, 3rd edition Brown TA Garland Science 2006

2 Gene Cloning: An Introduction Brown TA Stanley Thornes Publishers Limited 1995

3 Molecular Cloning: A Laboratory Manual, Vols 1- 3 J. Sambrook and D.W. Russel CSHL 2001

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