MTech Bio-analytical Techniques syllabus for 1 Sem 2020 scheme 20BBC15

BRIEF REVIEW OF ELECTROMAGNETIC SPECTRUM AND ABSORPTION OF RADIATIONS:

Theory of spectroscopy, absorption by organic molecules, choice of solvent and solvent effects, modern instrumentation – design and working principle. Applications of UV-Visible spectroscopy (qualitative and quantitative analysis). Principles of vibrational spectroscopy, frequency and factors influencing vibrational frequency, instrumentation and sampling techniques, interpretation of spectra, applications in biology. FT-IR-theory and applications, Attenuated Total Reflectance (ATR). Raman Spectroscopy, theory, instrumentation, and applications to biology. Discussions with Case studies.

FUNDAMENTAL PRINCIPLES OF NMR:

Instrumentation, solvents, chemical shift, and factors affecting chemical shift, spin-spin coupling, coupling constant, and factors influencing the value of coupling constant, spin-spin decoupling, proton exchange reactions, FT-NMR, 2D -NMR, NMDR, NOE, NOESY, COSY and applications in Pharmacy, interpretation of spectra, C13 NMR Introduction, Natural abundance, C13 NMR Spectra and its structural applications. Discussions with Case studies.

BASIC PRINCIPLES AND INSTRUMENTATION OF ION FORMATION AND TYPES:

Fragmentation processes and fragmentation pattern, Chemical ionization mass spectroscopy (CIMS), Field Ionization Mass Spectrometry (FIMS), Fast Atom Bombardment MS (FAB MS), Matrix Assisted laser desorption / ionization MS (MALDI-MS), GC-MS. LC-MS. MS-MS. Discussions with Case studies

INTRODUCTION TO X-RAY:

Generation of X-rays, X-ray diffraction, Bragg’s law, X-ray powder diffraction, interpretation of diffraction patterns and applications. Single crystal diffractions of biomolecules. Fibre diffraction. Neutron diffraction. XAFS. ORD Principle, Plain curves, curves with cotton effect, octant rule and its applications with example, circular dichroism and its relation to ORD. Discussions with Case studies

CHROMATOGRAFIC TECHNIQUES:

Classification of chromatographic methods based on mechanism of separation: paper chromatography, thin layer chromatography, ion exchange chromatography, column chromatography and affinity chromatography – technical questions and applications. Gas Chromatography: Theory and principle, column operation, instrumentation, derivatisation methods and applications. HPLC, LC-MS and applications in HPTLC. Discussions with Case studies.

Course outcomes:

At the end of the course the student will be able to:

• Demonstrate strong basics in principles of Analytical techniques
• Tackle live problems in various spheres of biological sciences

Question paper pattern:

The SEE question paper will be set for 100 marks and the marks scored will be proportionately reduced to 60.

• The question paper will have ten full questions carrying equal marks.
• Each full question is for 20 marks.
• There will be two full questions (with a maximum of four sub questions) from each module.
• Each full question will have sub question covering all the topics under a module.
• The students will have to answer five full questions, selecting one full question from each module.

Textbooks

1 Fundamentals of Bioanalytical Techniques and Instrumentation SabariGoshal& A K Srivastava PHI 2009

2 Principles of Instrumental Analysis by, 4th Edition Donglas A. Skoog, James, J. Leary Saunders College Publishing, Philadelphia 1992

Reference Books

1 Practical Pharmaceutical Chemistry, 4thEdition A. H. Beckett & J. B. Stenlake Academic Press 1988

2 Instrumental Methods of Chemical Analysis B. K. Sharma Goel Publishing House Meerut 2000

3 Biochemical Methods of Analysis SarojDua&Neera Garg Alpha Science 2010