Experiments
1 Preparation of buffers and biochemical reagents.
2 Estimation of proteins by Lowry’s and Bradford methods
3 Methods in genomic DNA/plasmid Isolation, Quantification of nucleic acids by agarose electrophoresis/Spectrophotometric methods
4 Quantification of nucleic acids by agarose gel-electrophoresis/ Spectrophotometric methods
5 Amplification of DNA by PCR.
6 Isolation and screening of microbes for Enzymes/Organic acids/secondary metabolites(antibiotics)/nitrogen fixing
7 Cell differentiation by gram staining
8 Isolation of Enzymes/organic acids (from suitable sources)
9 Perform bioassays like, Enzyme activity, specific activity, Antibiogram
10 Enzyme Kinetic Parameters: Km, Vmax and Kcatter
11 Optimization of biotic and abiotic parameters for enzyme production in batch fermentation
12 Batch growth kinetics of microbes
Course outcomes:
At the end of the course the student will be able to: