MTech Metabolic Engineering syllabus for 2 Sem 2020 scheme 20BBC254

Module-1 INTRODUCTION AND METABOLIC REGULATION 0 hours

INTRODUCTION AND METABOLIC REGULATION:

Introduction: Importance of metabolic engineering and its multidisciplinary nature. An overview of Cellular Metabolism, Transport Processes, Passive Transport, Facilitated Diffusion, Active Transport, Fueling Reactions, Fermentative Pathways, Glycolysis, TCA Cycle and Oxidative Phosphorylation, Anaplerotic Pathways, Catabolism of Fats, Organic Acids, and Amino Acids, Biosynthetic Reaction, Biosynthesis of Amino Acids, Biosynthesis of Nucleic Acids, Fatty Acids.

Module-2 METABOLIC FLUX AND APPLICATIONS OF METABOLIC FLUX ANALYSIS 0 hours

METABOLIC FLUX AND APPLICATIONS OF METABOLIC FLUX ANALYSIS:

Metabolic flux analysis and its application, Methods for experimental determination of metabolic flux by isotope dilution method. Production of Glutamic Acid and regulation by Bacteria, Calculation of Theoretical Yields, Metabolic Flux Analysis of Lysine Biosynthetic Network in C. glutamicum, Metabolic Flux Analysis of Specific Deletion Mutants of C. glutamicum, Metabolic Fluxes in Mammalian Cell Cultures, Determination of Intracellular Fluxes, Application of Flux Analysis to the Design of Cell Culture Media.

A d v e r t i s e m e n t
Module-3 REGULATION OF METABOLIC PATHWAYS 0 hours

REGULATION OF METABOLIC PATHWAYS:

Regulation of Enzymatic Activity, Overview of Enzyme Kinetics, Simple Reversible Inhibition Systems, Irreversible Inhibition, Allosteric Enzymes: Cooperativity, Regulation of Enzyme Concentration, Control of Transcription Initiation, Control of Translation, Global Control: Regulation at the Whole Cell Level, Regulation of Metabolic Networks, Branch Point Classification, Coupled Reactions and the Role of Global Currency Metabolites.

Module-4 METABOLIC ENGINEERING IN PRACTICE 0 hours

METABOLIC ENGINEERING IN PRACTICE:

Enhancement of Product Yield and Productivity, Ethanol, Amino Acids, Solvents, Extension of Substrate Range, Metabolic Engineering of Pentose Metabolism for Ethanol Production, Cellulose-Hemicellulose Depolymerization, Lactose and Whey Utilization, Sucrose Utilization, Starch Degrading Microorganisms, Extension of Product Spectrum and Novel Products, Antibiotics, Polyketides, Vitamins, Biopolymers, Biological Pigments, Hydrogen, Pentoses: Xylitol, Improvement of Cellular Properties, Prevention of Overflow Metabolism, Alteration of Substrate Uptake, Maintenance of Genetic Stability.

Module-5 BIOSYNTHESIS OF METABOLITES AND BIOCONVERSIONS 0 hours

BIOSYNTHESIS OF METABOLITES AND BIOCONVERSIONS:

Primary metabolites: Alteration of feedback regulation, limiting of accumulation of end products, resistant mutants. Secondary metabolites: Precursor effects, prophage, idiophase relationship, enzyme induction, feedback repression, catabolic repression, important groups of secondary metabolic enzymes, phosphotransferase, ligases oxidoreductases, oxygenases, carboxylases.Advantages of bioconversions, specificity, yields. Factors important to bioconversions, regulation of enzyme synthesis, permeability co metabolism, conversion of insoluble substrates.

 

Course outcomes:

After studying this course, students will be able to:

  • Demonstrate strong basics in metabolic engineering
  • Develop and design different metabolic pathways to understand the cell regulatory events

 

Question paper pattern:

The SEE question paper will be set for 100 marks and the marks scored will be proportionately reduced to 60.

  • The question paper will have ten full questions carrying equal marks.
  • Each full question is for 20 marks.
  • There will be two full questions (with a maximum of four sub questions) from each module.
  • Each full question will have sub question covering all the topics under a module.
  • The students will have to answer five full questions, selecting one full question from each module.

 

Textbook/ Textbooks

1 Metabolic Engineering year Principles and Methodologies Gregory N. Stephanopoulos, Aristos A. Aristidou, Jens Nielsen Academic Press 1998

2 Control of metabolic process A.C. Bowdenand M.L. Cardens Plenum Publisher 1991

 

Reference Books

1 Bioprocess engineering basic concepts M.L. Shuler and Kargi Pearson hall 1992

2 Fermentation and enzyme Technology Wang D I C Cooney C I Demain A L John Willey 1992

3 Scale-up Methods in Chemical Engineering Johnson and Thring Johnson and Thring 2006

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