Introduction to Protein Structure:
The Building Blocks, Motifs of Protein Structure, Protein folding Molecular Recognition: Protein-protein interactions, Protein-DNA interactions, The Thermodynamics of Binding like thermal stability and specificity of Macromolecular Recognition, Transcription, translation, and post-translational modifications of proteins
Concepts, principles and applications of various expression systems for protein and enzyme production. The principles and applications of the most current purification systems used in bioseparations.
Protein design principles, Student design proposals, Protein engineering by directed evolution and rational design, Directed Evolution Strategy- Phage Display Systems, Cell Surface Display Systems, Cell Free Display System, Alternative Scaffolds, Combinatorial Enzyme Engineering, Protein Engineering using noncanonical amino acids
Mutant selection and identifications, and establishment of mutant library for protein engineering, Enzyme and Biosensor engineering, Antibody engineering, Engineering of Therapeutic Proteins , In vitro synthetic enzymatic biosystems for bio-manufacturing of insulin, and other industrial enzymes Protein Arrays/ Protein Chips and their application, 2D Gel Electrophoresis and its application Mass Spectrometry and Protein Identification, Proteomics Databases Proteomics Analysis Tools
Course outcomes:
At the end of the course the student will be able to: Apprehend the concepts of protein engineering techniques and their applications
• Analyze the various types of protein structures and modifications
• Apply the knowledge of Protein engineering technology to produce novel proteins with pharmaceutical and industrial significance.
• Evaluate the biosafety, ethical and quality issues of various protein design technologies.
Question paper pattern:
The SEE question paper will be set for 100 marks and the marks scored will be proportionately reduced to 60.
• The question paper will have ten full questions carrying equal marks.
• Each full question is for 20 marks.
• There will be two full questions (with a maximum of four sub questions) from each module.
• Each full question will have sub question covering all the topics under a module.
• The students will have to answer five full questions, selecting one full question fromeach module.
Textbook/ Textbooks
1 Strategies for the Design of Novel Proteins:Computer Methods in Protein Modeling Jiri Novotny Academic Press 1996
2 Protein Engineering and Design Sheldon J. Park, Jennifer R. Cochran Press 2009
Reference Books
1 Phage Display Systems for Protein Engineering A. Ernst and S. S. Sidhu CRC 2009
2 Cell-Free Display Systems for Protein Engineering P. A. Barendt and C. A. Sarkar CRC 2009