MTech Concepts In Biotechnology syllabus for 1 Sem 2020 scheme 20BBI12

Introduction:

Macromolecules; Carbon chemistry; Proteins: Structure, folding, catalysis; Nucleic acids: DNA & RNA; storage and transfer of genetic information; Lipids: membranes, structure & function; Carbohydrate chemistry, energy storage, building blocks.

Cell Biology:

Eukaryotic and Prokaryotic cells, plant and animal cells, structure of nucleus, mitochondria, ribosomes, Golgi bodies, lysosomes, endoplasmic reticulum, chloroplast, vacuoles; Cell cycle and cell division: Different phases of cell cycle, cell division: Mitosis and meiosis. Mendelian law of inheritance: Monohybrid and dihybrid inheritance, law of segregation and independent assortment; Gene Interaction; Multiple alleles, supplementary and complementary genes, epistasis. Identification of genetic material: classical experiments; chromosome structure and organization, chemical composition of chromatin, structural organization of nucleosomes, heterochromatin, Polytene and lamp-brush chromosomes, human chromosomes, chromosomal disorders.

Microbiology:

Scope and History of microbiology, Introduction to the structure and functions of microorganism: Bacteria, Viruses, Fungi and Protozoan’s. Microscopy and microbial techniques: Study of microscopes; sterilization techniques: Heat, steam, Radiation, Filtration and chemical methods; Pure culture techniques: Serial Dilution, Streak, Spread, Pour Plate. Immune System, Innate and adaptive immunity, antigens and antibodies; types of immune response, hypersensitivity. Humoral immunity: B-lymphocytes, Immunoglobulin classes, Major Histocompatibility Complex (MHC). Cell mediated immunity. Thymus derived lymphocytes (T-cells), Antigen presenting cells (APC); Immunity to infection, Cytokines.

Agri BT:

Role of Micorbes in agriculture, Biopesticides, Bio fertilizers (Nitrogen fixing microbes), GM crops. Plant metabolic engineering and industrial products: Molecular farming for the production of industrial enzymes, biodegradable plastics, antibodies, edible vaccines. Metabolic engineering of plants for the production of fatty acids, industrial oils, flavonoids etc. Basic aspects of Food & Nutrition.

Industrial BT:

Industrially important Microorganisms, Preservation techniques, Different media for fermentation, basic structure of fermenter and different types. Types of fermentation processes (surface, submerged, and solid state) and their products (ethanol, citric acid, lactic acid, enzymes, antibiotics) Biological treatment of waste water, primary, secondary and tertiary treatments. Bio indicators, Bioremediation of xenobiotic compounds, Bioleaching of minerals from ores, Biosorption of toxic metals. Solid waste management. Biofuel production from agricultural wastes.

Course outcomes:

At the end of the course the student will be able to:

• Demonstrate strong fundamentals in principles of Biotechnology.
• Apply the theoretical principles for specific case studies and societal issues.

Question paper pattern:

The SEE question paper will be set for 100 marks and the marks scored will be proportionately reduced to 60.

• The question paper will have ten full questions carrying equal marks.
• Each full question is for 20 marks.
• There will be two full questions (with a maximum of four sub questions) from each module.
• Each full question will have sub question covering all the topics under a module.
• The students will have to answer five full questions, selecting one full question from each module.

Textbook/ Textbooks

1 Cell Biology, Genetics, Evolution and Ecology P S Verma, V R Agarwal, New Publisher, Delhi 2007

2 Industrial Microbiology Prescott and Dunn, Industrial Microbiology Macmillan 1982

Reference Books

1 Cell and Molecular Biology De Robertis EDP and De Robertis Jr. EMF, Wippincott, Williams and Willkins publisher, 2001.

2 Plant biotechnology. K. Lindsey and M.G.K. Jones Agriculture, Prentice hall, New Jersey 1989

3 Biodegradation and Detoxification of Environmental Pollutants, Munnecke DM, Johnson LM and others CRC Press 1982