17BT43 Molecular Biology syllabus for BT

INTRODUCTION & REPLICATION OF DNA:

Chromosomal theory of heredity, genes and their location. Genetic code, Information flow in biological systems: central dogma, updated central dogma. Structures and forms of nucleic acids – DNA and RNA. Replication of DNA, structure and function of DNA polymerases, models of replications in prokaryotes, mechanism of DNA replication and enzymes involved.

TRANSCRIPTION:

Structure and function of RNA polymerases (prokaryotes & eukaryotes), mechanism of transcription in prokaryotes and eukaryotes, transcription factors, post-transcriptional processing (RNA editing, siRNA, splicing, poly A tail and 5’ capping), transcription inhibitors.

TRANSLATION:

Mechanism of translation, activation of amino acid initiation, elongation and termination of protein synthesis. Post translational modification and protein targeting, protein splicing. Differences between prokaryotic and eukaryotic protein synthesis, inhibitors of translation.

GENE EXPRESSION IN PROKARYOTES & EUKARYOTES:

Regulation of gene expression in prokaryotes: Operon model, gal, lac, trp Operons; positive versus negative regulation. Regulation of eukaryotic gene expression, transcriptional control, homeobox in the control of developments in insects and vertebrates.

GENETIC RECOMBINATION, MUTATION & GENE MAPPING:

Genetic recombination in bacteria and viruses, site specific recombination, transposons and insertion sequences; Retroviruses. DNA damage & Repair, Mutation, Role of recombination and transposition in evolution; gene mapping techniques.

Course outcomes:

After studying this course, students will be able to:

• Explain replication, transcription and translation processes with underlying differences in prokaryotic and eukaryotic systems.
• Elaborate importance of genetic recombination with special reference to bacterial system.
• Outline DNA damage and repair mechanisms

REFERENCE BOOKS

1. Statistical methods in Bioinformatics by Warren J. Ewens, Gregory R. Grant, Springer 2nd edition, 2006.

2. An Introduction to Biostatistics by P. S. S. Sundar Rao and J. Richard, Prentice Hall of India, publications, 4th edition, 2006.

3. Biostatistics: A foundation for Analysis in the Health sciences by Wayne W. Daniel, John 7 th edition, 2000.

4. A Textbook of Molecular Biology by S.M Gopinath, Archers & Elevators International Publishing House India. 1st Edition. 2014.

5. Fundamentals of Biostatistics by Veer BalaRastogi, Ane Books India.

TEXT BOOKS

1. Essentials of Molecular Biology by David Freifelder, Narosa Pub. House.

2. Molecular Biology of the Cell by Alberts et al., Garland Publishing.

3. Principles of Gene manipulation and Genomics by Primrose, Oxford University Press.

4. Molecular Biology of the Gene by James D Watson et al., Pearson Education