Experiments
1 Study of Laboratory Instruments.
2 Media preparation, Preparation of plates and tubes.
3 Pure culture techniques (Streak, pour and spread - plates).
4 Enumeration of microbes by Plate count and haemo-cytometer.
5 Acid Fast Staining 6 Gram staining, Capsule staining, and endospore and flagella staining.
7 Staining of fungi.
8 Characterization of bacteria by Biochemical Tests: IMViC, Starch hydrolysis, carbohydrate fermentation, Catalase, Urease, hydrogen sulphide, Nitrate reduction.
9 Isolation of actinomycetes and rhizobium and their identification.
10 Determination of bacterial motility:
11 Growth curve studies.
12 Antibiotic sensitivity tests.
13 Milk reduction and alkaline phosphatase test.
14 Experiment related to MPN test
Note: Minimum 12 experiments are to be conducted
Course Outcomes:
At the end of the course the student will be able to:
Conduct of Practical Examination:
1. All laboratory experiments are to be included for practical examination.
2. Breakup of marks and the instructions printed on the cover page of answer script to be strictly adhered by the examiners.
3. Students can pick one experiment from the questions lot prepared by the examiners.
4. Change of experiment is allowed only once and 15% Marks allotted to the procedure part to be made zero.
Reference Books:
1. Microbiology: A Lab Manual by Cappuccino Pearson education, 2007
2. Lab Math by Dany Spencer Adams, IK Intl. Pub house.
3. Lab Ref by JaineRoskams& Linda Rodgers IK Intl.Pub house.
4. Case-Microbiology: An Introduction by Gerard J. Tortora, Berdell R. Funke, Christine L. 11thEdition- Pearson publications.
5. Laboratory Manual Of Microbiology And Biotechnology by Aneja K.R. Medtec, 2014