Experiments
1 pH measurements, volume / weight measurements, concentration units, sensitivity. Specificity, precision, accuracy, preparation of buffers of constant strength.
2 Titration of amino acids with acids & bases.
3 Qualitative tests for carbohydrate and lipids.
4 Qualitative tests for amino acids and proteins.
5 Estimation of blood sugar by Folin method and by O-toluene method.
6 Estimation of inorganic phosphate by Fiske-Subbarao method.
7 Estimation of amino acid by Ninhydrin method.
8 Estimation of total cholesterol from Serum.
9 Determination of saponification value and iodine value of lipids with error analysis.
10 Determination of acetyl value of a lipid with error analysis.
11 Estimation of urea by diacetylmonooxime method with error analysis.
12 Estimation of iron from hemoglobin with error analysis.
13 Estimation of Vitamin C by titrimetric method
14 Estmation of lactose in milk by DNS method
15 Estimation of uric acid in blood by Caraway’s method
16 Estimation of creatinine in serum and urine by Jaffe’s method
Note: Minimum 12 experiments are to be conducted
Course Outcomes:
At the end of the course the student will be able to:
Conduct of Practical Examination:
1. All laboratory experiments are to be included for practical examination.
2. Breakup of marks and the instructions printed on the cover page of answer script to be strictly adhered by the examiners.
3. Students can pick one experiment from the questions lot prepared by the examiners.
4. Change of experiment is allowed only once and 15% Marks allotted to the procedure part to be made zero.
Reference Books:
1. Modern Experimental Biochemistry by Rodney Boyer, Pearson Education.
2. Practical Biochemistry by Cole, Cambridge University Press.
3. Lab Ref by Jaine Roskams & Linda Rodgers, IK Intl. Pub. House.
4. Manual of Practical Biochemistry for medical students, 2nd edition, University Press