Experiments
1 Agglutination Technique: ABO typing
2 Differential counting of WBC
3 Isolation of lymphocytes from peripheral blood
4 Bacterial Agglutination reaction-Widal test (Tube / slide agglutination)
5 Radial Immunodiffusion (RID)
6 Ouchterlony Double Diffusion (ODD)
7 Rocket immunoelectrophoresis (RIEP)
8 Counter-current immunoelectrophoresis (CCIEP)
9 Dot ELISA
10 Coombs test
11 Complement fixation test
12 Isolation of Chicken Immunoglobulin (IgY) from Egg Yolk
13 Immunoglobulin purification from serum by IEC
14 Western blotting
Note: Minimum 12 experiments are to be conducted
Course Outcomes:
At the end of the course the student will be able to:
Conduct of Practical Examination:
1. All laboratory experiments are to be included for practical examination.
2. Breakup of marks and the instructions printed on the cover page of answer script to be strictly adhered by the examiners.
3. Students can pick one experiment from the questions lot prepared by the examiners.
4. Change of experiment is allowed only once and 15% Marks allotted to the procedure part to be made zero.
Reference Books:
1. Immunology by Kuby Cambridge University Press.
2. Immunology Laboratory Practices by Stryers
3. The Cell by Alberts Cambridge University Press