18BTL57 Biokinetics & Enzyme Technology Laboratory syllabus for BT

Experiments

1 Mixed Flow Reactor

2 Plug Flow Reactor

3 Isothermal Batch Reactor

4 RTD in Mixed Flow Reactor

5 RTD in Plug Flow Reactor

6 Isolation of enzymes

7 Determination of activity, Vmax & Km of α- amylase

8 Effect of Inhibitor on α- amylase 9 Time course of Amylase activity

10 Effect of pH on α- amylase

11 Effect of temperature on α- amylase

12 Enzyme Immobilization techniques and kinetics

13 Determination of specific activity of α- amylase

14 Isolation of papain(proteases) from papaya and assay of papain using calorimetric method

15 Effect of organic solvents on enzyme activity

16 Determination of molecular weight by SDS-PAGE

Note: Minimum 12 experiments are to be conducted Revised Bloom’s Taxonomy Level L1, L2, L3, L4, L5

Course outcomes:

At the end of the course the student will be able to:

• State and define the nature of the reaction, rate of the reaction, rate constant and enzyme activity.
• Compare the rate of reaction for different reactors; know the ethical responsibilities that come with conducting experiments and communicating data.
• Use the design equations for predicting the reactor performance.
• Compose the RTD data to identify non idealitites in different reactor configuration.

Conduct of Practical Examination:

1. All laboratory experiments are to be included for practical examination.

2. Breakup of marks and the instructions printed on the cover page of answer script to be strictly adhered by the examiners.

3. Students can pick one experiment from the questions lot prepared by the examiners.

4. Change of experiment is allowed only once and 15% Marks allotted to the procedure part to be made zero.

Reference Books:

1. Bioprocess Engineering by Shule and Kargi Prentice Hall.

2. Chemical Engineering Kinetics by Smith J.M. McGraw Hill.

3. Enzyme Technology by M.F. Chaplin and C. Bucke, Cambridge University Press, Cambridge.