Experiments
1 Isolation of genomic DNA (plant / microbial sources)
2 Isolation of plasmid DNA from bacteria
3 Agarose gel electrophoresis and quantification of nucleic acids (colorimetric)
4 Digestion and mapping of plasmid pUC18
5 Transformation and screeningof recombinants 6 Study of conjugation in bacteria
7 Amplification of DNA by PCR 8 Study of divisional stages in Mitosis & Meiosis.
9 Study of Polytene chromosomes 10 Isolation and fusion of plant protoplasts.
11 Callus Induction technique – Carrot/Beet root/ or any other material
12 Estimation of Lycopene from tomato fruits
13 Somatic Embryogenesis
14 Embryo/Endosperm Culture
15 Estimation of Anthocyanin from leaf /callus tissue 16 Cell viability assay- Tryphan blue Note: Minimum
12 experiments are to be conducted
Course outcomes:
At the end of the course the student will be able to:
Conduct of Practical Examination:
1. All laboratory experiments are to be included for practical examination.
2. Breakup of marks and the instructions printed on the cover page of answer script to be strictly adhered by the examiners.
3. Students can pick one experiment from the questions lot prepared by the examiners.
4. Change of experiment is allowed only once and 15% Marks allotted to the procedure part to be made zero.
Reference Books:
1. Experiments in Plant Tissue Culture by John H. Dodds & Lorin W. Robert.
2. Plant tissue Culture Theory and Practice by S.S. Bhojwani and M.K. Razdan, Elsevier.
3. Culture of Animal Cells. R. Ian Freshney and R. Alan., Liss. Inc. (1987).