17CH46 Instrumental Analysis [F.C] syllabus for CH

CHROMATOGRAPHY:

Classification of chromatographic methods based on the mechanism of separation. Column Chromatography: Adsorption and partition, theory, preparation, procedure and methods of detection. Thin Layer Chromatography: Theory, preparation, procedures, detection of compounds. Paper Chromatography: Theory, different techniques employed, filter papers used, qualitative and quantitative detection .Counter – current extraction, solid phase extraction techniques, gel filtration

GAS CHROMATOGRAPHY:

Introduction, fundamentals, instrumentation, columns: preparation and operation, detection, dramatization. . HPLC: Principles and instrumentation, solvents and columns, detection and applications, HPTLC: Theory and principle, instrumentation, elution techniques.

SPECTROSCOPY:

Introduction, electromagnetic spectrum, absorbance laws and limitations, instrumentation-design and working principle, chromophore concept, auxochromes, Wood-Fisher rules for calculating absorption maximum, applications of UV-Visible spectroscopy . IR spectroscopy: Basic principles-Molecular vibrations, vibrational frequency, factors influencing vibrational frequencies, sampling techniques, instrumentation, interpretation of spectra, FT-IR, theory and applications.

MASS SPECTROSCOPY:

Theory, ionization techniques: electron impact ionization, chemical ionization, field ionization, fast atom bombardment, plasma desorption, fragmentation process: types of fission, resolution, GC/MS, interpretation of spectra and applications for identification and structure determination.

NMR:

Theory, instrumentation, chemical shift, shielding and deshielding effects, splitting of signals, spin-spin coupling, proton exchange reactions, coupling constant (J), nuclear over Hauser effect (NOE), 13CNMR spectra and its applications, 2D-NMR, COSY and applications.

COURSE OUTCOME:

The appreciable knowledge will be gained by the students in the Modern Analytical Techniques and can apply the theories involved in the Analysis of various bulk drugs and their formulations. The students will also be in a position to apply their knowledge in developing the new methods for the determination and validate the procedures.

GRADUATE ATTRIBUTES:

1. Problem analysis.

2. Design/development of solutions.

QUESTION PAPER PATTERN:

• The question paper will have ten questions.
• Each full question is for 16 marks.
• There will be two full questions (with a maximum of four sub questions) from each module.
• Each full question will have sub questions covering all the topics under a module.
• The students will have to answer five full questions, selecting one full question from each module.

TEXT BOOKS

1. Instrumental Methods of Chemical Analysis by B.K Sharma

2. Organic Spectroscopy by Y.R Sharma

REFERENCE BOOKS:

1. Text book of Quantitative Chemical Analysis by Vogel’s A.I.

2. Organic Spectroscopy by William Kemp