BETCK105G Emerging Applications of Biosensors syllabus for CSE Stream Physics Group

INTRODUCTION TO BIOSENSORS

Introduction to biosensor, General components of biosensor, Biomolecules in biosensors such as enzyme, DNA, antigen antibody, protein, Classification of biosensors based on principle: amperometric, potentiometric biosensors, optical, acoustic, piezoelectric, and calorimetric biosensors, scope of biosensors and its limitations.

BASIC DESIGN AND TRANSDUCER

Design Considerations: calibration, dynamic Range, signal to noise, sensitivity, selectivity, Interference recognition. Transduction membrane protein sensors: ion channels, Types of Transducer, Optical; Fiber Optic, ECL, Surface Plasmon Resonance, Electro chemical; FET, Impedance, Piezoelectric; Cantileaver,

APPLICATIONS OF BIOSENSORS IN HEALTH AND ENVIRONMENT

Biosensors and diabetes management, Microfabricated biosensors and point-of-care diagnostics systems, Noninvasive biosensors in clinical analysis; Surface plasmon resonance and evanescent wave biosensors, Biosensor in cancer and HIV early diagnosis.

APPLICATIONS OF BIOSENSORS IN FOOD AND AGRICULTURE INDUSTRY

Detection of product content, allergic components, pathogens, pesticide residues. Monitoring of raw material conversions. Detection of crop diseases, pathogens in plants, Detection of soil nutrients, pesticide and its residual detection.

APPLICATIONS OF NANOMATERIALS IN BIOSENSORS

Nano Materials in biosensors; Carbon based Nano Material, Metal oxide and nano particle, Quantum dots, Role of nano material in Signal Amplifications, Detection and Transducer Fabrication.

Course outcome (Course Skill Set)

At the end of the course the student will be able to:

CO1 Classify types of biosensors based on principle

CO2 Able to differentiate different types of transducers based on their physicochemical characteristics

CO3 Apply bio sensing techniques in health, environment, agriculture and food industry.

CO4 Use biomaterial and nanomaterials in biosensors for signal amplification, Detection and Transducer Fabrication

Assessment Details (both CIE and SEE)

• The weightage of Continuous Internal Evaluation (CIE) is 50% and for Semester End Exam (SEE) is 50%.
• The minimum passing mark for the CIE is 40% of the maximum marks (20 marks out of 50).
• The minimum passing mark for the SEE is 35% of the maximum marks (18 marks out of 50).
• A student shall be deemed to have satisfied the academic requirements and earned the credits allotted to each subject/ course if the student secures not less than 35% (18 Marks out of 50) in the semester-end examination(SEE), and a minimum of 40% (40 marks out of 100) in the sum total of the CIE (Continuous Internal Evaluation) and SEE (Semester End Examination) taken together.

Continuous Internal Evaluation(CIE):

Two Unit Tests each of 30 Marks (duration 01 hour)

• First test after the completion of 30-40 % of the syllabus
• Second test after completion of 80-90% of the syllabus

One Improvement test before the closing of the academic term may be conducted if necessary. However best two tests out of three shall be taken into consideration

Two assignments each of 10 Marks

The teacher has to plan the assignments and get them completed by the students well before the closing of the term so that marks entry in the examination portal shall be done in time. Formative (Successive) Assessments include Assignments/Quizzes/Seminars/Course projects/Field surveys/ Case studies/ Hands-on practice (experiments)/Group Discussions/ others. The Teachers shall choose the types of assignments depending on the requirement of the course and plan to attain the Cos and POs. (to have a less stressed CIE, the portion of the syllabus should not be common /repeated for any of the methods of the CIE. Each method of CIE should have a different syllabus portion of the course). CIE methods /test question paper is designed to attain the different levels of Bloom’s taxonomy as per the outcome defined for the course.

The sum of two tests, two assignments, will be out of 100 marks and will be scaled down to 50 marks

Semester End Examination(SEE):

Theory SEE will be conducted by University as per the scheduled timetable, with common question papers for the subject (duration 03 hours)

• The question paper shall be set for 100 marks. The medium of the question paper shall be English/Kannada). The duration of SEE is 03 hours.
• The question paper will have 10 questions. Two questions per module. Each question is set for 20 marks.
• Students have to answer 5 full questions, selecting one full question from each module. The student has to answer for 100 marks and marks scored out of 100 shall be proportionally reduced to 50 marks.
• There will be 2 questions from each module. Each of the two questions under a module (with a maximum of 3 sub-questions), should have a mix of topics under that module.

Suggested Learning Resources:

Text Books:

1. Jeong-Yeol Yoon, Introduction to Biosensors, Springer-Verlag New York Ed. 2016

2. Mohammed Zourob, Recognition Receptors in Biosens; Publisher: Springer-Verlag New York Ed. 2010

Reference Books:

1. Zvi Liron, Novel Approaches in Biosensors and Rapid Diagnostic Assays; Publisher: Springer US Ed..2001

2. Pierre R. C, and Loïc J.B, Biosensor Principles and Applications, , CRC Press, 2019